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following mutagenesis at two sites near the catalytic pocket and the application of peroxidase-coated yeast

Karla Ilić Đurđić, Raluca Ostafe, Olivera Prodanović, Aleksandra Đurđević Đelmaš, Nikolina Popović, Rainer Fischer, Stefan Schillberg, Radivoje Prodanović

《环境科学与工程前沿（英文）》 2021年第15卷第2期 doi: 10.1007/s11783-020-1311-4

摘要： Abstract • Mutations in Lignin peroxidase Trp171 environment improved azo dyes degradation. • Expression on yeast cell surface and cell lysis allowed reusability of biocatalyst. • Aga2-LiP chimeric variants were characterized. The enzymatic degradation of azo dyes is a promising alternative to ineffective chemical and physical remediation methods. Lignin peroxidase (LiP) from Phanerochaete chrysosporium is a heme-containing lignin-degrading oxidoreductase that catalyzes the peroxide-dependent oxidation of diverse molecules, including industrial dyes. This enzyme is therefore ideal as a starting point for protein engineering. Accordingly, we subjected two positions (165 and 264) in the environment of the catalytic Trp171 residue to saturation mutagenesis, and the resulting library of 104 independent clones was expressed on the surface of yeast cells. This yeast display library was used for the selection of variants with the ability to break down structurally-distinct azo dyes more efficiently. We identified mutants with up to 10-fold greater affinity than wild-type LiP for three diverse azo dyes (Evans blue, amido black 10B and Guinea green) and up to 13-fold higher catalytic activity. Additionally, cell wall fragments displaying mutant LiP enzymes were prepared by toluene-induced cell lysis, achieving significant increases in both enzyme activity and stability compared to a whole-cell biocatalyst. LiP-coated cell wall fragments retained their initial dye degradation activity after 10 reaction cycles each lasting 8 h. The best-performing mutants removed up to 2.5-fold more of each dye than the wild-type LiP in multiple reaction cycles.

关键词： Bioremediation Enzyme immobilization Protein engineering Yeast surface display.

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Cell surface protein engineering for high-performance whole-cell catalysts

Hajime Nakatani,Katsutoshi Hori

《化学科学与工程前沿（英文）》 2017年第11卷第1期页码 46-57 doi: 10.1007/s11705-017-1609-3

摘要： Cell surface protein engineering facilitated by accumulation of information on genome and protein structure involves heterologous production and modification of cell surface proteins using genetic engineering, and is important for the development of high-performance whole-cell catalysts. In this field, cell surface display is a major technology by exposing target proteins, such as enzymes, on the cell surface using a carrier protein. The target proteins are fused to the carrier proteins that transport and tether them to the cell surface, as well as to a secretion signal. This paper reviews cell surface display systems for prokaryotic and eukaryotic cells from the perspective of carrier proteins, which determine the number of displayed molecules, and the localization, size, and direction ( or terminal anchoring) of the passengers. We also discuss advanced methods for displaying multiple enzymes and a new method for the immobilization of whole-cell catalysts using adhesive surface proteins.

关键词： cell surface engineering surface display whole-cell catalysts bioprocess

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Use of dry yeast cells as a cheap nitrogen source for lactic acid production by thermophilic

Kim Yng Ooi, Jin Chuan Wu

《化学科学与工程前沿（英文）》 2015年第9卷第3期页码 381-385 doi: 10.1007/s11705-015-1534-2

摘要： Dry yeast cells (DYC) were used as a cheap nitrogen source to replace expensive yeast extract (YE) for L-lactic acid production by thermophilic . Cassava starch (200 g·L ) was converted to L-lactic acid by simultaneous saccharification and fermentation using WCP10-4 at 50 °C in the presence of 20 g·L of DYC, giving 148.1 g·L of L-lactic acid at 27 h with a productivity of 5.5 g·L ·h and a yield of 92%. In contrast, 154.4 g·L of lactic acid was produced at 24 h with a productivity of 6.4 g·L ·h and a yield of 96% when equal amount of YE was used under the same conditions. Use of pre-autolyzed DYC at 50 °C for overnight slightly improved the lactic acid titer (154.5 g·L ) and productivity (7.7 g·L ·h ) but gave the same yield (96%).

关键词： L-lactic acid thermophilic strain Bacillus coagulans dry yeast cells autolysis fermentation

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SCRaMbLE drive application of synthetic yeast genome

Jin Jin, Yuan Ma, Duo Liu

《化学科学与工程前沿（英文）》 2018年第12卷第4期页码 832-834 doi: 10.1007/s11705-018-1749-0

摘要：

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我国信息显示关键材料发展战略研究

彭寿,洪伟 ,秦旭升 ,蒋文玖 ,黄毅

《中国工程科学》 2022年第24卷第4期页码 85-93 doi: 10.15302/J-SSCAE-2022.04.009

摘要： Liquid crystal display and organic light-emitting diode display: Present status and future perspectivesMini-LED and Micro-LED: Promising candidates for the next generation display technology [J].Chinese Academy of Engineering project “Development Strategy of Key Materials for Information Display

关键词：信息显示显示关键材料自主可控产业链供应链

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我国新型显示关键材料发展战略研究

高伟男,毕勇,刘新厚,许祖彦

《中国工程科学》 2020年第22卷第5期页码 44-50 doi: 10.15302/J-SSCAE-2020.05.007

摘要：

新型显示作为电子信息领域为数不多的千亿美元级产业，是信息化、智能化时代我国战略性新兴产业重点发展方向之一。本文针对新型显示关键材料与工艺在新型显示技术和产业发展进程中的战略性、支撑性和保障性作用开展论述，梳理了具有代表性的新型显示关键材料的研究现状与发展趋势，凝练了我国相关领域在材料技术和应用技术方面存在的问题，提出了我国新型显示关键材料发展目标和重点方向。研究建议：抓牢新一轮显示技术升级与产业转型的重大机遇，从国家经济发展全局的高度、全产业链生态的角度出发，以整机应用为牵引，以核心材料为突破口，打造新型显示创新平台，构建高效的技术创新体系；聚合优势力量，突破产业化发展中的核心共性关键材料与工艺问题，完善产业协同创新生态。从技术、市场、资金、政策等多个角度完成了新型显示创新平台的可行性分析，以期支持我国新型显示产业“从跟随到引领”的跨越式发展。

关键词：新型显示有机发光二极管微米级发光二极管激光显示关键材料

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激光显示技术发展的现状和趋势

高伟男,许祖彦,毕勇,袁园

《中国工程科学》 2020年第22卷第3期页码 85-91 doi: 10.15302/J-SSCAE-2020.03.013

摘要：

随着信息技术的不断发展，信息化时代已经来临，显示作为人机信息交互环节最重要的信息输出终端，已经遍及人们生活的各个方面。当今时代显示技术层出不穷，都以实现超高分辨率、大尺寸、真色彩的极致画面显示，满足人们追求极致美好视觉的需求为发展方向，目标就是实现高保真图像再现。激光显示是采用三基色激光作为显示光源的新型显示技术，可以实现双高清（几何/ 颜色）、大色域、高观赏舒适度的“冲击人眼视觉极限”高保真图像再现，被视为显示产业转型升级的重要方向之一。本文立足新形势下显示产业自主可控安全发展的需求，详细论述了激光显示技术和产业特点，梳理和总结了本领域发展现状、趋势和短板问题，凝练出激光显示产业发展路线图，建议我国应抓住这一大好机遇，加大关键核心技术攻关，自主可控加速将激光显示产业发展壮大，支撑下一代显示产业的高端市场，并开拓出激光显示规模产业集群。

关键词：激光显示，双高清，大色域，高观赏舒适度

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工程师突破超高分辨率显示屏技术瓶颈

Mitch Leslie

《工程（英文）》 2021年第7卷第7期页码 897-898 doi: 10.1016/j.eng.2021.06.002

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新型潜艇作战系统TMA功能设计

任克明,潘悦,王岩

《中国工程科学》 1999年第1卷第3期页码 31-34

摘要：

文章在新型潜艇全综合、分布式作战系统的体系结构下，以声纳信息作为主要信息源，综述了纯方位TMA、多信息融合TMA、方位-多普勒TMA、噪声能量-方位联合估距、与匹配场声源定位相结合等几种实现TMA的方法，在此基础上对TMA功能提出了新设想，初步提出了一种TMA的功能设计。该功能设计从作战系统的角度出发开发TMA功能，充分利用了多传感器获得的信息，以及人的主观判断，增强了TMA的能力，使它能够更好地为指挥员战术决策服务，为武器的发射控制提供更加精确的目标运动要素解算结果。与通常的TMA设计相比，该项设计能够识别目标机动，增加了人机交互功能，并设计了便于观看TMA结果、监视目标机动和执行交互式跟踪改进的TMA显示画面。

关键词：运动目标分析潜艇作战系统显示

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In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water of different treatment processes

Kaifeng RAO, Na LI, Mei MA, Zijian WANG

《环境科学与工程前沿（英文）》 2014年第8卷第1期页码 69-78 doi: 10.1007/s11783-013-0502-7

摘要： Effluents from wastewater treatment plant (WWTP) have been reported to have a broad spectrum of endocrine disrupting compounds (EDCs). The majority of studies have focused on the occurrence of estrogenic activity, while ignoring nuclear hormone receptors (NRs) pathways. In the present study, a battery of in vitro yeast bioassays and a cell bioassay, including antagonistic and agonistic effects on estrogen receptor (ER), androgen receptor (AR), progesterone receptor (PR), estrogen-related receptor (ERR) and aryl hydrocarbon receptor (AHR), were conducted to evaluate the removal efficiencies of EDCs by different treatment processes of a WWTP located in Beijing. Estrogenic, anti-estrogenic, anti- androgenic, anti-progesteronic, anti-ERR and the activation of AHR activities were detected in samples from all treatment processes and the receiving water. The concentration of estrogenic contaminants with estradiol (E ) equivalent concentrations ranged from 0.82 × 10 to 3.54 × 10 g E -EQ·L . The concentration of anti-estrogenic contaminants with 4-hydroxytamoxifen (4-OHT) equivalent concentrations ranged from 1.24 × 10 to 2.36 × 10 g 4-OHT-EQ·L . The concentration of anti-androgenic contaminants ranged from 2.21 × 10 to 3.52 × 10 g flutamide-EQ·L . The concentration of anti-progesteronic contaminants ranged from 3.15 × 10 to 2.71 × 10 g RU486-EQ·L . The concentration of anti-ERR contaminants ranged from 7.09 × 10 to 6.50 × 10 g 4-OHT-EQ·L . The concentration of AHR activators ranged from 1.7 × 10 to 3.4 × 10 g TCDD-EQ·L . These processes including secondary clarifier, coagulation, as well as coal and sand filtration could eliminated 67.2% of estrogenic contaminants, 47.0% of anti-estrogenic contaminants, 98.3% of anti-androgenic contaminants, 88.4% of anti-progesteronic contaminants, 65.4% of anti-ERR contaminants and 46.9% of AHR activators. WWTP effluents contain multiple receptor disruptors may have very complex adverse effects on exposed organisms.

关键词： waste water in vitro recombinant yeast assay ethoxyresorufin-O-deethylase (EROD) receptor endocrine

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An Exploration of Surface Integrity Remanufacturing for Aeroengine Components

Qiao Xiang,Yong He,Ting-hong Hou

《工程管理前沿（英文）》 2016年第3卷第2期页码 107-114 doi: 10.15302/J-FEM-2016025

摘要： Surface integrity is the major factor impacting on the operation quality, service life and reliability of the aeroengine components. The surface integrity of aeroengine component is damaged by the failures such as crack, deformation, oxidation, corrosion, erosion, and microstructural degeneration. It adopts advanced remanufacturing technologies to restore or improve the surface integrity and regenerate these high value parts. This paper firstly puts forward the concept, namely surface integrity remanufacturing for aeroengine components, and its connotation. The key remanufacturing technologies have been developed to repair the components with surface damages. Ultimately, some application examples of surface integrity remanufacturing technologies as well as their effects in aeroengine maintenance are introduced. The discarded components have been reused and their service lives have been extended and their reliability has been increased by implementing surface integrity remanufacturing. It has realized “The Repaired Components Outpacing the New Ones”, material saving, energy saving, and emission reduction.

关键词： aeroengine component surface integrity remanufacturing surface integrity remanufacturing

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Review on mechanism and process of surface polishing using lasers

Arun KRISHNAN, Fengzhou FANG

《机械工程前沿（英文）》 2019年第14卷第3期页码 299-319 doi: 10.1007/s11465-019-0535-0

摘要： Laser polishing is a technology of smoothening the surface of various materials with highly intense laser beams. When these beams impact on the material surface to be polished, the surface starts to be melted due to the high temperature. The melted material is then relocated from the ‘peaks to valleys’ under the multidirectional action of surface tension. By varying the process parameters such as beam intensity, energy density, spot diameter, and feed rate, different rates of surface roughness can be achieved. High precision polishing of surfaces can be done using laser process. Currently, laser polishing has extended its applications from photonics to molds as well as bio-medical sectors. Conventional polishing techniques have many drawbacks such as less capability of polishing freeform surfaces, environmental pollution, long processing time, and health hazards for the operators. Laser polishing on the other hand eliminates all the mentioned drawbacks and comes as a promising technology that can be relied for smoothening of initial topography of the surfaces irrespective of the complexity of the surface. Majority of the researchers performed laser polishing on materials such as steel, titanium, and its alloys because of its low cost and reliability. This article gives a detailed overview of the laser polishing mechanism by explaining various process parameters briefly to get a better understanding about the entire polishing process. The advantages and applications are also explained clearly to have a good knowledge about the importance of laser polishing in the future.

关键词： laser polishing surface roughness process parameters mechanism

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Tribological study on the surface modification of metal-on-polymer bioimplants

《机械工程前沿（英文）》 2022年第17卷第2期 doi: 10.1007/s11465-022-0682-6

摘要： The tribological performance of artificial joints is regarded as the main factor of the lifespan of implanted prostheses. The relationship between surface roughness and coefficient of friction (COF) under dry and lubricated conditions is studied. Results show that under dry test, friction coefficient is not reduced all the time with a decrease in surface roughness. On the contrary, a threshold of roughness value is observed, and frictional force increases again below this value. This critical value lies between 40 and 100 nm in S_a (roughness). This phenomenon is due to the transfer of friction mechanisms from abrasion to adhesion. Under wet test, COF always decreases with reduction in surface roughness. This result is mainly attributed to the existence of a thin layer of lubricant film that prevents the intimate contact of two articulating surfaces, thus greatly alleviating adhesion friction. Furthermore, surface texturing technology is successful in improving the corresponding tribological performance by decreasing friction force and mitigating surface deterioration. The even-distribution mode of texturing patterns is most suitable for artificial joints. By obtaining the optimal surface roughness and applying texturing technology, the tribological performance of polymer-based bioimplants can be greatly enhanced.

关键词： artificial joints surface roughness friction surface texturing

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非平面电极双稳态电润湿显示器件研究 Regular Papers-Research Articles

Han ZHANG, Xue-lei LIANG

《信息与电子工程前沿（英文）》 2019年第20卷第9期页码 1289-1295 doi: 10.1631/FITEE.1800167

摘要：双稳态电润湿显示器仅在状态切换时耗能，在状态稳定时无耗能，是一种非常有潜力的低能耗电子纸显示技术。本文设计和实现了通过常规光刻工艺制备一种非平面电极结构的双稳态电润湿器件。该器件不仅具备视频响应速度，而且有潜力实现可控的像素灰阶。与现有平面电极结构双稳态电润湿器件相比，该新型电极结构可实现更低驱动电压和更高对比度。

关键词：双稳态电润湿；非平面；控制电极；低电压；高对比度

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Development of surface reconstruction algorithms for optical interferometric measurement

Dongxu WU, Fengzhou FANG

《机械工程前沿（英文）》 2021年第16卷第1期页码 1-31 doi: 10.1007/s11465-020-0602-6

摘要： Optical interferometry is a powerful tool for measuring and characterizing areal surface topography in precision manufacturing. A variety of instruments based on optical interferometry have been developed to meet the measurement needs in various applications, but the existing techniques are simply not enough to meet the ever-increasing requirements in terms of accuracy, speed, robustness, and dynamic range, especially in on-line or on-machine conditions. This paper provides an in-depth perspective of surface topography reconstruction for optical interferometric measurements. Principles, configurations, and applications of typical optical interferometers with different capabilities and limitations are presented. Theoretical background and recent advances of fringe analysis algorithms, including coherence peak sensing and phase-shifting algorithm, are summarized. The new developments in measurement accuracy and repeatability, noise resistance, self-calibration ability, and computational efficiency are discussed. This paper also presents the new challenges that optical interferometry techniques are facing in surface topography measurement. To address these challenges, advanced techniques in image stitching, on-machine measurement, intelligent sampling, parallel computing, and deep learning are explored to improve the functional performance of optical interferometry in future manufacturing metrology.

关键词： surface topography measurement optical interferometry coherence envelope phase-shifting algorithm

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标题作者时间类型操作

following mutagenesis at two sites near the catalytic pocket and the application of peroxidase-coated yeast

Karla Ilić Đurđić, Raluca Ostafe, Olivera Prodanović, Aleksandra Đurđević Đelmaš, Nikolina Popović, Rainer Fischer, Stefan Schillberg, Radivoje Prodanović

期刊论文

Cell surface protein engineering for high-performance whole-cell catalysts

Hajime Nakatani,Katsutoshi Hori

期刊论文

Use of dry yeast cells as a cheap nitrogen source for lactic acid production by thermophilic

Kim Yng Ooi, Jin Chuan Wu

期刊论文

SCRaMbLE drive application of synthetic yeast genome

Jin Jin, Yuan Ma, Duo Liu

期刊论文

我国信息显示关键材料发展战略研究

彭寿,洪伟 ,秦旭升 ,蒋文玖 ,黄毅